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RNA isolated both from Vero E6 or C6/36 cells derived virus was reverse transcribed and the ensuing cDNA amplified employing SuperScript III reverse transcriptase in the One-Action RT-PCR System with Platinum Taq DNA Polymerase (Invitrogen)

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Ideal preparations had been attained utilizing a subsequent protocol: Clarified cell society supernatants had...
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The increased expression of GSK-3 led to the downregulation of -catenin and, subsequently, cyclin D1 and c-myc. These conclusions indicated that the knockdown of galectin-three inhibits the expression of Wnt downstream effectors including -catenin, cyclin D1 and c-myc at the mRNA and protein ranges

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TCF4/ -catenin responsive elements are identified in the promoter of the human MDR1 gene,...