centration. Each qRT-PCR was performed in triplicate. Real-time RT-PCR was performed in September Rat Gene Profiling Changes plates using the Verso SYBR Green AD mean Body weight Body length Liver weight Spleen weight Heart weight Fat pad weight Plasma glucose Urea Free Fatty Acids UN mean order Ligustilide Adjusted p-value Results Phenotypic Assessment For each of the Glycerol Total Protein Lipase C-Peptide Triglycerides IGF- Expression Analysis Expression profiling was done for liver, white adipose fat, and skeletal muscle tissue from young adult male rats born to either AD or UN dams. In each group there were eight biological replicates, all of which were raised postnatally on an ad libitum diet. Expression profiles were determined using the Illumina RatRef- Phenotypic measurements relevant to metabolic syndrome measured for each of the eight animals in each treatment group at day tissue in the day September Rat Gene Profiling Changes AD mean Body weight Body length Total Fat Fat pad weight Leptin Insulin C-Peptide Plasma glucose Free Fatty Acids Liver weight IGF- UN mean Adjusted p-value NS undernutrition during pregnancy. It has also been suggested that PEPCK and Discussion Animals at postnatal day Phenotypic measurements relevant to metabolic syndrome measured for each of the eight animals in each treatment group at day Quantitative RT-PCR Analysis of Selected Genes. In order to confirm the expression data derived from array analysis, seven genes, FABPGene Symbol ADRA Genbank Accession Number NM_ Microarray Expression QRT-PCR Expression Fold change for the QRT-PCR data and the Illumina expression data is compared for each of the seven genes from the Illumina arrays that were analyzed by QRTPCR. Fold changes for the RT-PCR reflect directly the DDCt values between the two groups after normalization. doi: Rat Gene Profiling Changes Analysis of the gene categories and associated pathways associated with differentially expressed genes has revealed some relevant changes that potentially impact on the metabolic phenotype. Insulin and IGF-The insulin and IGF- Glucose Metabolism Mitochondrial Activity The array analysis indicates a number of changes in the expression of genes that could affect the efficiency of electron transport and ATP generation in the mitochondrion in liver from UN animals. Transcripts of NADH dehydrogenase subunits of complex I of the electron transport chain are all reduced as is quinolate phosphoribosyltransferase required for the synthesis of NAD, the electron carrier. In addition, the ATP synthase that generates ATP from the electron gradient is 
reduced by a similar amount. Changes are also observed in peptidylprolyl isomerase D that controls the mitochondrial permeability transition pore and TIMSeptember METABOLIC AREA Gys SYMBOL GENE IDENTIFICATION % DEXP FUNCTION interference with GLUT Carbohydrate and Glucose metabolism activator of NHF- Fat and Lipid Metabolism increased transport of acylCoA from cytosol to mitochondrion shown to regulate energy expenditure and lipolysis isomerase involved in the conversion of lanosterol to cholesterol aspartate aminotransferase in aa catabolism and malate shuttle Amino Acid Metabolism September Rat Gene Profiling Changes Protein turnover adapts proteosome to counteract stress-induced proteotoxicity SYMBOL Cand METABOLIC AREA Ribosomes Apoptosis September Rat Gene Profiling Changes Mitochondrion and Electron Transport Chain SYMBOL BC METABOLIC AREA Transcription September Summarizes the intere
