YsoPC (16:0/22:6(4Z, 7Z, 10Z, 13Z, 16Z, 19Z)) (p = 0.0008), LysoPC (19:0/0:0) (p = 0.0036), LysoPC (20:1(9Z)/0:0) (p = 0.0331), and LysoPE (0:0/22:0) (p = 0.001) in APP/PS1 mice (Figure S2). Our final results are constant with these of preceding studies. five. Conclusions In this study, we observed that capsaicin rescued the behavioral cognitive deficits of APP/PS1 mice. Congo red staining showed that capsaicin decreased -amyloid deposits in the brain tissues in APP/PS1 mice. Gut microbiota profiling indicated that the capsaicin-treated mice harbored greater relative abundances of Akkermansia, Faecalibaculum, Unclassified_f_Atopobiaceae, and Gordonibacter, although the vehicle-treated APP/PS1 mice harbored larger relative abundances of Adlercreutzia, Peptococcaceae, Alistipes, Oscillibacter, and Erysipelatoclostridium.Complement C3/C3a Protein Accession Notably, A. muciniphila was observed to become significantly increased within the capsaicin-treated mice. The metabolome profiling of serum samples showed that capsaicin-treated mice had a higher amount of Trp metabolism as well as a reduce degree of lipid metabolism when compared with vehicle-treated APP/PS1 mice. The proof of A. muciniphila and Trp metabolites highlighted that capsaicin-enriched diets might mitigate the incidence and development of AD by altering gut microbiome and serum metabolome, plus a.TROP-2 Protein Source muciniphila and Trp metabolism may well be feasible approaches for ameliorating AD.Supplementary Components: The following supporting information could be downloaded at: https: //mdpi/article/10.PMID:25147652 3390/nu15010118/s1, Figure S1: Relative abundance of gut microbiota in APP/PS1 mice (APP/PS1_CTRL: n = 7; APP/PS1_CP: n = 7). (A) Alpha diversity evaluation of gut bacterial diversity and richness from various mouse groups. (B) Bar plot displaying changes within the relative abundance of distinctive bacterial classes in the genus level in APP/PS1 mice treated with vehicle or capsaicin; Figure S2: Significant effects of capsaicin treatment were noted with respect to lipid metabolism in APP/PS1 mice (all groups: n = 5). Author Contributions: J.L., X.L. and L.Z. conceived and planned the experiments; X.Y., G.H. and F.J. offered capsaicin; Z.D. ready the suspension of capsaicin; J.L. carried out animal experiments; J.L. analyzed the data and wrote the manuscript with support from W.Z.; L.Z. and X.L. edited the manuscript. All authors have read and agreed towards the published version on the manuscript. Funding: This study was supported by China Agriculture Analysis System of MOF and MARA (grant quantity: CARS-24-E-03) and the 2115 Talent Development Program of China Agricultural University. Institutional Evaluation Board Statement: All experimental procedures were authorized by the respective Institutional Animal Care and Use Committees of China Agricultural University (Issue quantity: Aw20902202-4-1).Nutrients 2023, 15,14 ofInformed Consent Statement: Not applicable. Data Availability Statement: Sequencing reads along with the genome assembly are offered in NCBI beneath bioproject PRJNA895588. The fecal metabolite data are offered in MetaboLights under MTBLS6342. Acknowledgments: The authors would prefer to thank Yong Xue, Daotong Li, and Yuan Li for important reading of this manuscript. We appreciate the technical assistance offered by Majorbio Bio-Pharm Technology Co., Ltd. Conflicts of Interest: The authors declare that they’ve no conflict of interest.
Intrinsically Disordered Proteins (IDPs) are a widespread class of diverse proteins characterized by lack of a fixed 3D structure [1]. IDPs are w.
