caput epididymis tissue (Figure 4E,F).Various AgesAnimals 2021, 11,11,FOR PEER Critique Animals 2021, x9 of 9 of 15Figure 4. The expression levels of NR1D1 and NR4A2 mRNA and proteins in adult yak (4 years old) HPG tissues. A and expression of NR1D1 and NR4A2 mRNA, respectively, in yak HPG tissues, measured making use of qPCR. (C,D), The expression B, The expression of NR1D1 and NR4A2 mRNA, respectively, in yak HPG tissues, measured using qPCR. C and D, The of NR1D1 NR1D1 and NR4A2 protein, respectively, tissues, measured applying Western blot. (E,F), The IOD and F, The expression ofand NR4A2 protein, respectively, in yak HPGin yak HPG tissues, measured utilizing Western blot. Evalues of NR1D1 and NR4A2 proteins in yak HPG in yak respectively. respectively. The expression levels NR4A2 mRNA and IOD values of NR1D1 and NR4A2 proteinstissues, HPG tissues, The expression levels of NR1D1 and of NR1D1 and NR4A2 protein in the hypothalamus were made use of had been CYP2 Activator manufacturer controls. The expression expression of -actin was used as an ATM Inhibitor site endogenous mRNA and protein within the hypothalamus because the utilized as the controls. Theof -actin was employed as an endogenous control. , represent extremely substantial distinction vs. control (p 0.01). control. , represent important distinction vs. manage (p 0.05), , represent really important difference vs. handle (p 0.01).Figure 4. The expression levels of NR1D1 and NR4A2 mRNA and proteins in adult yak (four years old) HPG tissues. (A,B), The3.5. Expression Patterns of NR1D1 and NR4A2 mRNA and Protein in Yak Testicular Tissues with Distinct Ages The mRNA and protein expression levels of NR1D1 and NR4A2 were also examined in yak testicular tissues from yaks of different ages (Figure 5). The results showed thatAnimals 2021, 11,substantial (p 0.01, Figure 5A,B). The expression amount of NR4A2 mRNA was also sign icantly distinct in testicular tissues of yaks at eight years of age (p 0.01). The outcomes showe that NR1D1 and NR4A2 proteins have been differentially expressed in yak testicular tissues distinctive ages (Figure 5C,D). Utilizing the testicular tissues of yaks at 2 years of age as a co ten measured trol, the highest expression levels of NR1D1 and NR4A2 proteins have been also of 15 testicular tissues of 6-year-old yaks (p 0.01, Figure 5E,F).Figure five. Expression patterns of NR1D1 and NR4A2 mRNA and protein in yak testicular tissues in animals of distinct ages.Figure five. Expression patterns of NR1D1 and NR4A2 respectively, inprotein in yak testicularanimals of diverse ages, distinctive (A,B), The expression of NR1D1 and NR4A2 mRNA, mRNA and yak testicular tissues from tissues in animals of and B, making use of qPCR. (C,D), NR1D1 and NR4A2 mRNA, respectively, in yak testicular tissues from animals ages. A detectedThe expression ofThe expression of NR1D1 and NR4A2 protein, respectively, detected employing Western blot in yak of distinctive ages, detected using qPCR. C and D, The expression of NR1D1 and NR4A2 protein, testicular tissuesdetected applying Westreproductive axis tissues. (E,F), The IOD worth of NR1D1 and NR4A2 protein, respectively, in respectively, from yaks of ern blot differentreproductive axis tissues. E and F, The IODmRNA and protein in testicular tissues of 2-year-old yaks had been testicular in yak ages. The expression levels of NR1D1 and NR4A2 value of NR1D1 and NR4A2 protein, respectively, in employed yaks of diverse ages. of -actin was utilized as an endogenous control. , represent significant distinction vs. control tissues from as controls. The expression The expressi
