Elongation for 7 min at 72 . Amplification products were visualized in eight polyacrylamide (Figure 1B). A DNA sample from a colony having a higher amount of infection with nosematosis, confirmed by microscopy, was utilised as a constructive control. Animals 2021, 11, Microscopic examination of intestinal contents was carried out individually in 103 of 7 2892 bees from every single colony. The intestine of a single bee was rubbed with 1 mL of distilled water, a drop in the resulting suspension was examined below a microscope at a magnification of 400X using a Biomed microscope (Russia).DNA sample from a colony with a high amount of infection polyacrylamide (Figure 1B). Awith nosematosis, confirmed by microscopy, was employed as a constructive manage.Figure 1. Gel photos of PCR solutions: (A)–mtDNA COI-COII intergenic locus; (B)–16S rRNA gene fragment of N. apis (1) and N.1. Gel photos of PCR merchandise: A–mtDNA COI-COII intergenic (400 in macerated abdomen Figure ceranae (four); NC–negative handle; PC–positive handle; (C)–Nosema spore locus; B–16S rRNA gene suspension of adult honeybees. fragment of N. apis (1) and N. ceranae (4); NC–negative control; PC–positive control; C–Nosema spore (400X) in macerated abdomen suspension of adult honeybees.3. ResultsAnalysis of analyzed colonies3.belong to the evolutionary lineage C (allelic variant Q, Table 1). Outcomes Nosematosis was located in 50of the polymorphism of(42 positiveslocus N. apis that 30.four from the anaAnalysis from the 92 colonies the COI-COII for showed and 8–for N. ceranae). Microscopic examination of towards the evolutionarythe middle intestinesQ, Table bees from lyzed colonies belong the contents of lineage C (allelic variant of your 1). Nosematosis was identified in 50 of the 92 colonies (42 positives for N. apis and 8–for N. ceranae). of these colonies showed the presence of spores only in those colonies where the quantity Microscopic examination with the 1C). amplification item was large (Figurecontents of your middle intestines of the bees from these coloniesTable 1. Distribution of nosematosis in colonies belonging for the evolutionary lineages M (allelic variant PQQ of of nosematosis and C (allelic variant Q) in the territory of M Altyn Solok reserve. Table 1. Distribution the COI-COII)in colonies belonging for the evolutionary lineages the(allelic variant PQQ of theCOI-COII) and C (allelic variant Q) within the territory with the Altyn Solok reserve.Microscopic examination of intestinal contents was carried out individually in 10 bees from every single colony. The intestine of a single bee was rubbed with 1 mL of distilled water, a drop of your resulting suspension was examined beneath a microscope at a magnification of 400using a Biomed microscope (Russia). the polymorphism of the COI-COII locus showed that 30.four of theshowed the presence of spores only in those colonies ATP disodium Cancer exactly where the level of amplification product was large (Figure 1C).Evolutionary lineage Evolutionary Lineage M M C C In total: In total:N. of ColoniesN. of Colonies64 64 28 28 92N. of Colonies Infested N. of Colonies Infested N. of N. with Colonies Infested with with of Colonies Infested with N. apis N. ceranae N. apis N. ceranae 31 (48.four ) 7 (10.9 ) 31 (48.4 ) 7 (ten.9 ) 11 (39.3 ) 1 (3.6 ) 11(39.3 ) 1 (three.six ) 42 (45.7 ) 8 (eight.7 ) 42(45.7 ) eight (8.7 )Our spot sampling showed that about half with the colonies suffer from nosematosis. the sector exactly where the biggest number of samples was taken (39 colonies), N. apis was found In the sector exactly where 33 colonies and N. ceranae in 1 col.
