Ssion to non-human primates it was shown that the predominant primary HIV-1 targets are T lymphocytes [1,27]. These cells express high levels of CD4 and CCR5 and are depleted in the vagina of infected animals [28]. While our data did not reveal differences in the infection patterns of T cells between T/F and C/R HIV-1 variants, it is conceivable that some minor subpopulations of tissue cells, may be ML-281 differentially infected by these two groups of viruses 18325633 but produce too a small quantity of p24 to affect the bulk production of T cells and thus to be noticed. Nevertheless, taking into account all the above reservations regarding our experimental system, our data did not reveal any particular features of T/F HIV-1 variants that made them strikingly different from the reference HIV-1 variants used in the current experiments. For ultimate determination of whether there are HIV-1 quasispecies that preferentially overcome HIV gatekeeper mechanisms or whether HIV-1 particles are transmitted stochastically, further studies utilizing pairs of transmitted and nontransmitted HIV-1 variants from the same host are necessary. Results from a recent transmission pair study [29] indicate argued that HIV-1 transmission is not solely stochastic, but there are sequences that seem to be transmitted preferentially. In contrast, in a recent in vitro study [30] no differences were found between T/F and C/R HIV-1 in their transmission efficiency across cervical tissue or in the genetic diversity of these viruses before and after transmission. More studies are needed to understand the biological propertied of HIV-1 variants that transmit infection.Author 
ContributionsConceived and designed the experiments: JCG LM RJS JCK CO. Performed the experiments: MM AA JCG. Analyzed the data: JCG MM LM AA. Contributed reagents/materials/analysis tools: CO JCK TGE. Wrote the paper: JCG LM TGE RJS JCK CO AA MM.
Critically ill patients are 115103-85-0 admitted in intensive care units (ICU) following various conditions such as sepsis, trauma, pancreatitis, hemorrhagic shock, or surgery. All these conditions share common host-response characteristics, referred to as systemic inflammatory response syndrome (SIRS) [1]. Both sepsis and non-sepsis-related SIRS are characterized by an exacerbated inflammatory response [2], and mortality remains high, especially in the settings of severe sepsis and septic shock [3]. A common feature of these patientsresides in alteration of their immune status, termed as compensatory anti-inflammatory response syndrome (CARS), which is thought to render them more susceptible to nosocomial infections [4], and to lead to increased morbidity and mortality in the ICU [5]. To date, various immunotherapies have failed to prevent the consequences of SIRS/CARS in severely septic patients, and efforts are still needed to fully understand the effects of the inflammatory and anti-inflammatory processes on the immune status of these patients [2,6].NK Cells and Critically-Ill Septic PatientsAlthough monocytes from patients with SIRS or sepsis have been studied [7,8], NK cells have received much less analysis. NK cells, found within the bloodstream, are also abundant in some tissues such as the lung [9], an organ particularly prone to dysfunction in ICU patients [10]. Importantly, murine experiments have shown collectively a deleterious proinflammatory effect of NK cells [11?6]. In these models, NK cells were a major source of interferon (IFN)-c, a potent immuno-stimulatory cytok.Ssion to non-human primates it was shown that the predominant primary HIV-1 targets are T lymphocytes [1,27]. These cells express high levels of CD4 and CCR5 and are depleted in the vagina of infected animals [28]. While our data did not reveal differences in the infection patterns of T cells between T/F and C/R HIV-1 variants, it is conceivable that some minor subpopulations of tissue cells, may be differentially infected by these two groups of viruses 18325633 but produce too a small quantity of p24 to affect the bulk production of T cells and thus to be noticed. Nevertheless, taking into account all the above reservations regarding our experimental system, our data did not reveal any particular features of T/F HIV-1 variants that made them strikingly different from the reference HIV-1 variants used in the current experiments. For ultimate determination of whether there are HIV-1 quasispecies that preferentially overcome HIV gatekeeper mechanisms or whether HIV-1 particles are transmitted stochastically, further studies utilizing pairs of transmitted and nontransmitted HIV-1 variants from the same host are necessary. Results from a recent transmission pair study [29] indicate argued that HIV-1 transmission is not solely stochastic, but there are sequences that seem to be transmitted preferentially. In contrast, in a recent in vitro study [30] no differences were found between T/F and C/R HIV-1 in their transmission efficiency across cervical tissue or in the genetic diversity of these viruses before and after transmission. More studies are needed to understand the biological propertied of HIV-1 variants that transmit infection.Author ContributionsConceived and designed the experiments: JCG LM RJS JCK CO. Performed the experiments: MM AA JCG. Analyzed the data: JCG MM LM AA. Contributed reagents/materials/analysis tools: CO JCK TGE. Wrote the paper: JCG LM TGE RJS JCK CO AA MM.
Critically ill patients are admitted in intensive care units (ICU) following various conditions such as sepsis, trauma, pancreatitis, hemorrhagic shock, or surgery. All these conditions share common host-response characteristics, referred to as systemic inflammatory response syndrome (SIRS) [1]. Both sepsis and non-sepsis-related SIRS are characterized by an exacerbated inflammatory response [2], and mortality remains high, especially in the settings of severe sepsis and septic shock [3]. A common feature of these patientsresides in alteration of their immune status, termed as compensatory anti-inflammatory response syndrome (CARS), which is thought to render them more susceptible to nosocomial infections [4], and to lead to increased morbidity and mortality in the ICU [5]. To date, various immunotherapies have failed to prevent the consequences of SIRS/CARS in severely septic patients, and efforts are still needed to fully understand the effects of the inflammatory and anti-inflammatory processes on the immune status of these patients [2,6].NK Cells and Critically-Ill Septic PatientsAlthough monocytes from patients with SIRS or sepsis have been studied [7,8], NK cells have received much less analysis. NK cells, found within the bloodstream, are also abundant in some tissues such as the lung [9], an organ particularly prone to dysfunction in ICU patients [10]. Importantly, murine experiments have shown collectively a deleterious proinflammatory effect of NK cells [11?6]. In these models, NK cells were a major source of interferon (IFN)-c, a potent immuno-stimulatory cytok.
