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TLR7 monoclonal antibody (4F4)
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TLR7 is one of toll-like receptors which are type I transmembrane proteins involved in innate immunity by recognizing microbial conserved structures. These proteins are characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain, and act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. Flow cytometry analysis of TLR7 in THP-1 cells. The cell was stained at 2-5µg for 1×106 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TLR7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant human TLR7 protein. Flow cytometry analysis of TLR7 in THP-1 cells. The cell was stained at 2-5µg for 1×106 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TLR7 antibody (1:1000).1135219-23-6 MedChemExpress Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant human TLR7 protein.50-56-6 Purity
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| Alternative Name toll-like receptor 7 | Application ELISA, Flow Cytometry, WB | Clone 4F4 | Formulation Liquid.PMID:30422575 In PBS, pH 7.4, containing 0.02% sodium azide and 10% glycerol. | Host Mouse | Immunogen Recombinant human TLR7 (aa 451-500) purified from E. coli. | Isotype IgG1κ | Source Purified from hybridoma tissue culture supernatant. | Species Reactivity Human | UniProt ID Q9NYK1 | Unit of Measure (UM) µl
