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IL-1β (human), ELISA kit
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Sensitive – quantify down to 1 pg/mLEfficient – only 50 µl diluted sample required saving precious materialsSpecific – no cross-reactivity with related cytokinesResults from up to 40 samples in duplicate in just 3 hoursReady-to-use reagents and pre-coated plates reduce errors and save your time The IL-1β (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in The interleukin-1 (IL-1) cytokine family consists of IL-1α, IL-1β, and IL-1Rα (receptor antagonist) which are expressed primarily by endothelial cells and activated macrophages. IL-1α and IL-1β elicit pro-inflammatory cytokine production and cellular activation, and are the primary mediators of hypothalamic regulation that results in fever. IL-1α and IL-1βsignals are transmitted via a dual subunit IL-1 receptor composed of IL-1R1 and the IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP is vital to IL-1 signaling, as it recruits the IL-1 receptor-associated kinase (IRAK) to the receptor complex, initiating NF-κB pathway activation.{{51-98-9} medchemexpress|{51-98-9} Purity & Documentation|{51-98-9} In stock|{51-98-9} supplier}
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| Alternative Name Interleukin-1β | Application Colorimetric detection, ELISA | Application Notes For the quantitative determination of IL-1β in culture supernatant, plasma, serum, and urine of human origin.{{1478364-68-9} web|{1478364-68-9} Protocol|{1478364-68-9} Formula|{1478364-68-9} manufacturer} | Assay Time | Contents Standard, Standard Diluent Buffer, Coated 96-well plate, Conjugate, Streptavidin-HRP, Streptavidin-HRP Diluent, Wash Buffer Concentrate, TMB Solution, Stop Solution, Plate Sealers | Crossreactivity The following substances were tested and found to have no cross-reactivity: human IL-1α, IL-1ra, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-10, IFN-α, TGF-β, GM-CSF, G-CSF, OSM, MIP-1α, MIP-1β, LIF, MCP-1, G-CSF, RANTES, PF-4, βTG, GRO, IP-10, SCF, TNF-α, and TNF-β.PMID:31424801 | Sensitivity 1 pg/mL | Species Reactivity Human | Technical Info / Product Notes Cited samples:Cytokine ELISAs Cited Samples | UniProt ID P01584 | Wavelength 450 nm