ly various involving the high-yielding strain (H) and also the low-yielding strain (L) in the 3 modules of blue, brown, and bisque4. The results of correlation evaluation among two modules (Supplementary Figure S4) show that blue and brown, and blue and bisque4 are substantially negatively correlated, with correlation coefficients of – 0.7 and – 0.59, respectively. Brown and bisque4 are weakly correlated, with a correlation coefficient of 0.24. GO and KEGG enrichment evaluation on blue, brown, and bisque4.GO enrichment evaluation was carried out on genes inside the three modules of blue, brown, and bisque4, respectively (Supplementary Figure S5). The outcomes showed that genes in these three modules have been mainly enriched in catalytic activity and binding in the molecular functions; metabolic processes, cellular processes, and ADAM17 Inhibitor custom synthesis single-organism processes in the biological processes; and cell and cell parts within the cellular element. The three modules had the same GO enrichment outcomes, only the amount of genes was diverse. Furthermore, KEGG enrichment results (Supplementary Table S3) for the 3 modules were not exactly the same. The brown module (P 0.05) was primarily enriched in the metabolic pathways of glyceride, sulfur, and galactose; Nav1.2 drug non-homologous finish joining; and microbial metabolism in diverse environments (Fig. 3). The blue module (P 0.05) was mainly enriched in the metabolism and biosynthesis of various amino acids; metabolism of oxycarboxylic acid, and folate; biosynthesis of secondary metabolites, aminoacyl-tRNA, pantothenate, and CoA; microbial metabolism in diverse environments; basalResultsScientific Reports | Vol:.(1234567890)(2021) 11:18207 |doi.org/10.1038/s41598-021-97616-nature/scientificreports/Figure 1. Venn diagrams of DEGs. (a) Venn diagram of DEGs in the course of 3 cultivation periods of high-yielding strains; (b) Venn diagram of DEGs through three cultivation periods with the low-yielding strains; (c) Venn diagram of DEGs amongst high-yielding and low-yielding strains in 3 culture periods; (d) Venn diagram of DEGs in high-yielding strains, low-yielding strains, between high- and low-yielding strains. transcription factors, and so forth. (Fig. 4). The bisque4 module (P 0.05) was mostly enriched within the cell cycle; meiosis; DNA repair; mismatch repair; nucleotide excision repair; base excision repair; biosynthesis of terpenoid backbones, and unsaturated fatty acids; and fatty acid metabolism (Fig. 5). The KEGG enrichment benefits of your three modules have been significantly various, which was consistent together with the results on the module correlation evaluation. The genes related to triterpenoid anabolism in every module had been selected in accordance with KEGG annotation results of genes, and these genes together with the above gene’s expression correlation weight value amongst in the module have been best 10 had been selected (Supplementary Table S4). Those genes had been selected for GO and KEGG enrichment. GO enrichment (Supplementary Figure S6) showed that these chosen genes have been mainly concentrated in catalytic activity and binding within the molecular functions; metabolic processes, cellular processes, and single-organism processes within the biological processes; and cell and cell components in the cellular element. The enrichment of these 3 modules’ genes was still essentially precisely the same. Detailed GO facts of those three modules’ genes is displayed in Supplementary Tables S5. KEGG enrichment results of genes related to triterpenoid biosynthesis in each module (Supplementary Table S8
