Pigment through thin layer chromatography (TLC), Fouriertransform infrared spectroscopy (FT-IR), and
Pigment through thin layer chromatography (TLC), Fouriertransform infrared spectroscopy (FT-IR), and proton nuclear magnetic resonance (1 Htransform infrared spectroscopy (FT-IR), and proton nuclear magnetic resonance (1HNMR) analyses revealed the presence of antimicrobial pigment rodiginine derivatives NMR) analyses revealed the presence of antimicrobial pigment rodiginine derivatives in Streptomyces sp. BSE6.1 [25]. Nevertheless, the genome NOP Receptor/ORL1 Gene ID evaluation of strain BSE6.1 reveals the in Streptomyces sp. BSE6.1 [25]. However, the genome analysis of strain BSE6.1 reveals the presence of an undecylTrxR Gene ID prodigiosin gene cluster that is responsible undecylprodigiosin presence of an undecylprodigiosin gene cluster which is responsible forfor undecylprodigiproduction. For that reason, the the red red fraction of Streptomyces strain BSE6.1 [25] to be osin production. Thus,otherotherfraction of Streptomyces strain BSE6.1 [25] is but is however 13 elucidated and and identified through LC-MS, 13C NMR, HSQC, HMBC, and COSY information to be elucidated identified through LC-MS, C NMR, HSQC, HMBC, and COSY data to confirm the production of undecylprodigiosin or connected derivatives. to confirm the production of undecylprodigiosin or associated derivatives. Prior studies reported that Streptomyces longisporus, Streptomyces spectabilis [7,57], Previous research reported that Streptomyces longisporus, Streptomyces spectabilis [7,57], and Streptomyces variegatus make prodigiosin [16] (Table 1). However, some strains of and Streptomyces variegatus generate prodigiosin [16] (Table 1). Having said that, some strains of Streptomyces coelicolor generate either undecylprodigiosin [17,20,58] or a mixture of prodigStreptomyces coelicolor create either undecylprodigiosin [17,20,58] or even a mixture of prodiinine derivatives [59] (Table 1). Comparable to S. coelicolor [17,20,58,59], the first fraction of ginine derivatives [59] (Table 1). Similar to S. coelicolor [17,20,58,59], the very first fraction of red pigment eluted from Streptomyces strain BSE6.1 via TLC revealed the presence red pigment eluted from Streptomyces strain BSE6.1 by way of TLC revealed the presence of of methyl-3-propyl prodiginine and 2-methyl-3-butyl prodiginine in mass spectrometry methyl-3-propyl prodiginine and 2-methyl-3-butyl prodiginine in mass spectrometry evaluation but identified it as prodigiosin in 1 H NMR evaluation [25]. Methyl-3-propyl prodiganalysis but identified it as prodigiosin in 1H NMR evaluation [25]. Methyl-3-propyl prodiinine and 2-methyl-3-butyl prodiginine have been also identified in actinomycetes [60], nonginine and 2-methyl-3-butyl prodiginine were also identified in actinomycetes [60], nonactinomycetes bacteria including Pseudoalteromonas rubra [61], and Serratia marcescens [62]. actinomycetes bacteria including Pseudoalteromonas rubra [61], and Serratia marcescens [62]. These research recommend that some strains of Streptomyces produce either prodigiosin or These studies suggest that some strains of Streptomycesof prodiginine analogs. undecylprodigiosin, whereas some generate a mixture produce either prodigiosin or undecylprodigiosin, whereas someof strain BSE6.1 made a total of 7,528,288 reads. AssemWhole-genome sequencing generate a mixture of prodiginine analogs. bling these raw reads resulted in a single scaffold of 8.02 Mb with no extra-chromosomal content. Annotating the assembled genome of strain BSE6.1 indicated the presence of at the least 7157 protein-coding genes, 82 tRNA coding genes, three rRNA coding genes, and.
