ly distinct among the high-yielding strain (H) and also the low-yielding strain (L) inside the three modules of blue, brown, and bisque4. The outcomes of correlation analysis among two modules (Supplementary Figure S4) show that blue and brown, and blue and bisque4 are significantly negatively correlated, with correlation coefficients of – 0.7 and – 0.59, respectively. Brown and bisque4 are weakly correlated, having a correlation coefficient of 0.24. GO and KEGG enrichment analysis on blue, brown, and bisque4.GO enrichment analysis was carried out on genes inside the three modules of blue, brown, and bisque4, respectively (Supplementary Figure S5). The results showed that genes in these three modules have been primarily enriched in catalytic activity and binding in the molecular functions; metabolic processes, cellular processes, and single-organism processes TRPA manufacturer within the biological processes; and cell and cell components in the cellular element. The three modules had exactly the same GO enrichment final results, only the amount of genes was different. Furthermore, KEGG enrichment outcomes (Supplementary Table S3) for the three modules have been not the same. The brown module (P 0.05) was primarily enriched inside the metabolic pathways of glyceride, sulfur, and galactose; non-homologous end joining; and microbial metabolism in diverse environments (Fig. 3). The blue module (P 0.05) was mostly enriched within the metabolism and biosynthesis of several amino acids; metabolism of oxycarboxylic acid, and folate; biosynthesis of secondary metabolites, aminoacyl-tRNA, pantothenate, and CoA; microbial metabolism in diverse environments; basalResultsScientific Reports | Vol:.(1234567890)(2021) 11:18207 |doi.org/10.1038/s41598-021-97616-nature/scientificreports/Figure 1. Venn diagrams of DEGs. (a) Venn diagram of DEGs for the duration of 3 cultivation periods of high-yielding strains; (b) Venn diagram of DEGs through 3 cultivation periods in the low-yielding strains; (c) Venn diagram of DEGs among high-yielding and low-yielding strains in three culture periods; (d) Venn diagram of DEGs in high-yielding strains, low-yielding strains, in between high- and low-yielding strains. transcription aspects, and so on. (Fig. four). The bisque4 module (P 0.05) was mostly enriched within the cell cycle; meiosis; DNA repair; mismatch repair; nucleotide excision repair; base excision repair; biosynthesis of terpenoid backbones, and unsaturated fatty acids; and fatty acid metabolism (Fig. 5). The KEGG enrichment outcomes from the 3 modules had been substantially different, which was constant with all the benefits of the module correlation evaluation. The genes associated with triterpenoid anabolism in each and every module were chosen based on KEGG annotation results of genes, and these genes using the above gene’s expression correlation weight worth among within the module were prime ten had been chosen (Supplementary Table S4). These genes had been chosen for GO and KEGG enrichment. GO enrichment (Supplementary Figure S6) showed that these selected genes were mainly concentrated in catalytic activity and binding in the molecular functions; metabolic processes, cellular processes, and single-organism processes within the biological processes; and cell and cell components in the cellular component. The enrichment of these 3 modules’ genes was nonetheless basically the same. Detailed GO facts of these three modules’ genes is displayed in Supplementary Tables S5. KEGG enrichment outcomes of genes P2X1 Receptor manufacturer related to triterpenoid biosynthesis in every single module (Supplementary Table S8
