ly different amongst the high-yielding strain (H) along with the low-yielding strain (L) inside the 3 modules of blue, brown, and bisque4. The outcomes of correlation evaluation between two modules (MGAT2 list Supplementary Figure S4) show that blue and brown, and blue and bisque4 are substantially negatively correlated, with correlation coefficients of – 0.7 and – 0.59, respectively. Brown and bisque4 are weakly correlated, with a correlation coefficient of 0.24. GO and KEGG enrichment analysis on blue, brown, and bisque4.GO enrichment evaluation was carried out on genes within the three modules of blue, brown, and bisque4, respectively (Supplementary Figure S5). The outcomes showed that genes in these 3 modules have been mainly enriched in catalytic activity and binding inside the molecular functions; metabolic processes, cellular processes, and single-organism processes inside the biological processes; and cell and cell components within the cellular component. The three modules had the identical GO enrichment results, only the number of genes was distinctive. Moreover, KEGG enrichment benefits (Supplementary Table S3) for the three modules have been not precisely the same. The brown module (P 0.05) was primarily enriched inside the metabolic pathways of glyceride, sulfur, and galactose; non-homologous finish joining; and microbial metabolism in diverse environments (Fig. 3). The blue module (P 0.05) was mainly enriched in the metabolism and biosynthesis of different amino acids; metabolism of oxycarboxylic acid, and folate; biosynthesis of secondary metabolites, aminoacyl-tRNA, pantothenate, and CoA; microbial metabolism in diverse environments; basalResultsScientific Reports | Vol:.(1234567890)(2021) 11:18207 |doi.org/10.1038/s41598-021-97616-nature/scientificreports/Figure 1. Venn diagrams of DEGs. (a) Venn diagram of DEGs throughout 3 cultivation periods of high-yielding strains; (b) Venn diagram of DEGs through 3 cultivation periods from the low-yielding strains; (c) Venn diagram of DEGs in between high-yielding and low-yielding strains in 3 culture periods; (d) Venn diagram of DEGs in high-yielding strains, low-yielding strains, involving high- and low-yielding strains. transcription variables, etc. (Fig. 4). The bisque4 module (P 0.05) was mainly enriched in the cell cycle; meiosis; DNA repair; mismatch repair; nucleotide excision repair; base excision repair; biosynthesis of terpenoid backbones, and NTR1 custom synthesis unsaturated fatty acids; and fatty acid metabolism (Fig. five). The KEGG enrichment final results on the three modules were significantly different, which was consistent together with the final results of your module correlation analysis. The genes associated with triterpenoid anabolism in each and every module have been selected according to KEGG annotation benefits of genes, and those genes together with the above gene’s expression correlation weight worth among in the module have been best 10 have been selected (Supplementary Table S4). Those genes were selected for GO and KEGG enrichment. GO enrichment (Supplementary Figure S6) showed that these chosen genes had been mainly concentrated in catalytic activity and binding within the molecular functions; metabolic processes, cellular processes, and single-organism processes inside the biological processes; and cell and cell parts inside the cellular component. The enrichment of those 3 modules’ genes was nonetheless basically the same. Detailed GO information and facts of these three modules’ genes is displayed in Supplementary Tables S5. KEGG enrichment final results of genes related to triterpenoid biosynthesis in each and every module (Supplementary Table S8
