Ation with the MSCs, the pellets were cultured in vitro in chondrogenic medium with various concentrations of BMP7. CXCL15 Proteins MedChemExpress sodium pyruvate (Gibco Invitrogen), and 10 ng/mL human TGF1 (R D Systems, Wiesbaden, Germany). Culture time was 21 days. For analysis in the influence of BMP7 on the chondrogenesis of MSCs of rabbits, five, 10, 50, one hundred, or 200 ng/mL BMP7 (generous present from Genera Biotech, Zagreb, Croatia) was added with or devoid of ten ng/mL TGF1 to the culture medium. two.8. Collagen II ELISA Evaluation for Chondrogenic Differentiated MSC Aggregates. An enzyme-linked immunosorbent assay test for collagen II was performed on chondrogenically differentiated MSC aggregates. Pellets have been homogenized in 0.05 M acetic acid plus 0.five M NaCl (pH two.9-3.0), digested.
