Ed in oMSCs treated with yEVs. With respect to pluripotency markers at RNA level, a statistically substantial lower of pluripotency markers (Nanog and Oct4) in yMSCs treated with oEVs as well as a statistically A Disintegrin and Metalloprotease 22 Proteins medchemexpress considerable boost in oMSCs treated with yEVs have been obtained. Around the contrary, it was obtained with respect Vinculin expression. At proteomic level, a statistically substantial enhance of three isoforms of Lamin A/C in yMSCs treated with oEVs and statistically significant lower in oMSCs treated with yEVs were obtained. Summary/conclusion: MSC-derived EVs and their self-renewal capacity had been influenced by age, as reveled by alterations in the expression of self-renewal markers which include Nanog, Oct4, Vinculin and Lamin A/C. These findings are important towards the recognize the influence of the ageing on MSCs and to advance in the improvement EV-based therapies.PT06.Ex vivo expansion of umbilical cord blood-derived haematopoietic stem cells using extracellular vesicles of bone marrow stromal origin Corina Ghebes1; Jess Morhayim2; Marion Kleijer1; Bram van der Eerden3; Jeroen van de Peppel3; Eric Braakman2; Carlijn Voermans1 Department of Hematopoiesis, Sanquin Study, Amsterdam, The Netherlands; 2Department of Hematology, Erasmus University Medical Center, Rotterdam, The Netherlands; 3Department of Internal Medicine, Erasmus University Health-related Center, Rotterdam, The NetherlandsPT06.Functionality of bone marrow Mesenchymal stem cell-derived extracellular vesicles on self-renewal Juan Antonio Fafian Labora1; Miriam Morente-L ez2; Patricia D zBarreiro2; Pablo Fernandez-Pernas2; Mar Arufe Gonda1 Grupo de Terapia Celular y Medicina Regenerativa (TCMR-CHUAC), CIBER BBN/ISCIII, Instituto de Investigaci Biom ica de A Coru (INIBIC), Complexo Hospitalario Universitario de A Coru (CHUAC), SERGAS, Departamento de Ciencias Biom icas, Medicina y Fisioterapia, Facultade 1Grupo de Terapia Celular y Medicina Regenerativa (TCMRCHUAC), CIBER BBN/ISCIII, Instituto de Investigaci Biom ica de A Coru (INIBIC), Complexo Hospitalario Universitario de A Coru (CHUAC), SERGAS, Departamento de Ciencias Biom icas, Medicina y Fisioterapia, Facultade de Oza, Universidade da Coru (UDC), As Xubias, A Coru , Spain. Oza, Universidade da Coru (UDC), As Xubias, A Coru , Spain., A Coru , Spain; 2Grupo de Terapia Celular y Medicina Regenerativa (TCMR-CHUAC), CIBER BBN/ISCIII, Instituto de Investigaci Biom ica de A Coru (INIBIC), Complexo Hospitalario Universitario de A Coru (CHUAC), SERGAS, Departamento de Ciencias Biom icas, Medicina, A Coru , SpainBackground: Mesenchymal stem cells (MSCs) are hugely relevant for regeneration of mesoderm tissues. The promising part of MSCs in cellbased therapies and tissue engineering is ADAM Metallopeptidase Domain 7 Proteins custom synthesis restricted due to a decline pluripotency, proliferation and immunogenic possible with escalating donor age. Extracellular vesicles (EVs) have already been recognizing as potent automobiles of intercellular communication on diverse biological systems. MSC-derived EVs present benefits more than cell-based therapy since it eliminates the safety issues related with injection of MSCs in individuals and especially useful for enhancing recovery from several ailments. Here, the influence of ageing on self-renewal functionality MSC-derived EVs working with an in vitro model was studied. Solutions: Two age groups from bone marrow MSCs of male Wistar rats were used, old (three months old) and young (14 days old). EVs wereBackground: Ex vivo expansion of umbilical cord blood (UCB)-deri.
