Ltration. EVs had been characterized by Raman spectroscopy using a 532 nm laser and the Ebola Virus NP Proteins Accession spectra analysed by multivariate analysis. Gold nanoparticles conjugated with antibodies against plasmaSaturday, 05 Maymembrane proteins present around the EVs had been made use of to detect the EVs making use of SERS. Benefits: The EV spectra show characteristic bands for proteins, lipids and nucleic acids. Multivariate analysis shows a detectable distinction involving EV populations. SERS enables extra targeted detection based on precise proteins present on the EV surface, allowing phenotyping analysis in the population. Summary/Conclusion: This study shows that Raman spectroscopy is really a helpful strategy for characterization of EVs in a label-free manner. Further, SERS may be employed for targeted evaluation with the EVs by conjugating antibodies towards the metal nanoparticles, with Cyclin-Dependent Kinase 4 Inhibitor D Proteins Storage & Stability facile multiplexing. Funding: This study was funded by the EPSRC and MRC below grant number EP/L019559/1 (OPTIMA) and by the University of Edinburgh College of Medicine and Veterinary Medicine.PS08.Electrochemical and optical biosensing for the detection of cancer exosomes from breast cancer cells Silio Lima Moura; MercMart Maria Isabel Pividori Grup de Sensors i Biosensors, Departament de Qu ica, Universitat Aut oma de Barcelona, Barcelona, Spaincomposition and functions. Quantification of low concentrations of specific exosomes present in quite smaller volumes of clinical samples could lead to non-invasive cancer diagnosis and prognosis. Methods: Using droplet microfluidics, we encapsulated single exosome complexes tagged with an enzymatic reporter that produces fluorescent signal for detection. Benefits: Our droplet based single exosome counting immunoassays (droplet digital ExoELISA) strategy enables absolute counting of cancer-specific exosomes to attain unprecedented accuracy. Using a plasma sample of 10 , we have been capable to detect as few as five enzymelabelled exosome complexes ( 10-17 M). We demonstrated the application of the droplet digital ExoELISA platform in quantitative detection of exosomes straight in plasma samples from breast cancer individuals. Summary/Conclusion: We think our method could have the prospective for cancer early diagnostics and accelerate the discovery of clinical diagnostic cancer exosomal biomarkers.PS08.Virtual Biorepository (VBR): a web-based service for sharing biofluid-, tissue-, cell- as well as other bio-samples Neethu Shah1; Sameer Paithankar1; William Thistlethwaite1; Jorge Arango2; Yashar Kalani3; Julie Saugstad4; Theresa Lusardi4; Joseph Quinn4; Lori Chase5; Tushar Patel5; Andrew R. Jackson6; Sai Lakshmi Subramanian6; Matthew Roth6; Bob Carter7; Fred Hochberg8; Aleksandar Milosavljevic6 Baylor College of Medicine, Houston, USA; 2Phoenix Children’s Hospital, Phoenix, USA; 3Barrow Neurological Institute, Phoenix, USA; 4Oregon Health Science University, Portland, USA; 5Mayo Clinic, Jacksonville, USA; 6Department of Molecular Human Genetics, Baylor College of Medicine, Houston, USA; 7Department of Neurosurgery, Massachusetts Basic Hospital, Boston, MA, Boston, USA; 8UC San Diego, San Diego, USABackground: The identification of novel biomarkers represents a worldwide challenge not only for the improvement of early diagnostics, but additionally for patient monitoring and for the evaluation of the efficiency of a therapeutic strategy. Exosomes are nano-sized and cup-shaped vesicles, that are at the moment below intensive study as possible diagnostic biomarkers for a lot of well being issues, such as c.
