Hiocyanate, it’s essential to separate and enrich thiocyanate from raw milk, so that the interference of other elements of raw milk in the detection method can be excluded. Among the available approaches for thiocyanate enrichment, solidphase extraction methods have mainly been studied. Al-Saidi et al. [6] established a headspace sorptive solid-phase microextraction (HS-SPME) method for the extraction of thiocyanate and cyanide. Below the optimized circumstances, the LOD and LOQ had been 0.34 and 1.two mmol/L, respectively. Da Silva et al. [7] separated thiocyanates using capillary electrophoresis. The ranges of LOD and LOQ had been 0.03.04 and 0.05.07 mg/L. Though the electromigration approach is economical and environmentally friendly, it requires a high degree of professionalism in the operator and isn’t very easily promoted in factories. Lu et al. [8] extracted thiocyanate ions inside the aqueous phase with complexes of Hg2 into methyl isobutyl ketone plus the LOD was 1.33 ng/mL. On the other hand, the presently reported techniques for thiocyanate determination suffer from the disadvantages of cumbersome pretreatment, low sensitivity, several PSB-603 Antagonist influencing factors, and high price. For that reason, a much more rapid, sensitive, straightforward, and economical technique for the enrichment and detection of thiocyanate in raw milk is essential. An aqueous two-phase system (ATPS) is formed primarily by the partitioning of two immiscible solutions. In some fields, ATPS is often a novel option method to conventional solvent extraction. ATPS has effectively been employed for the extraction and purification of peptides [9], polysaccharides [10], enzymes [11], heavy metals [12], proteins [13], amino acids [14], cells [15], and cytochromes [16], and has also been utilized for the separation and enrichment of neutral, anionic, and cationic ions [17]. In recent years, some novel, low-cost, and effective ATPSs have appeared which are distinct from typical polymer/salt and ionic liquid/salt systems. These ATPSs have received increasing focus resulting from high extraction efficiency, rapidly phase separation, low viscosity, mild environmental effects, and recyclability, which include alcohol/inorganic salt systems [18], propanol/inorganic salt systems [19], acetonitrile/inorganic salt systems [20], and so on. These ATPSs allow the recovery of tiny organic molecules in the ATPSs by evaporation and crystallization, creating it simple to separate the extract from the wealthy organic phase, therefore minimizing the price of extraction and enrichment, and simplifying the subsequent production course of action for quick application in downstream production [11]. In analytical applications, such systems conveniently exclude the possible interference of background within the determination of target substances. Since the introduction of ion chromatography (IC) by Shapiro et al. [21] in 1975, the process has come to be the preferred method for the determination of modest inorganic and organic ions. Thienpont et al. [22] reported that IC could determine the total sodium and potassium concentrations in human serum. Charles et al. [23] proposed a system of ion chromatography-mass spectrometry (IC-MS) to determine bromate ions in water. Fernandes et al. [24] utilised IC combined with UV detection to detect 4 bisphosphonates in pharmaceuticals or bulk materials. Now, the determination of thiocyanate in the industry is largely primarily based around the spectrophotometric strategy, which can be based on the principle that SCN- can produce blood-red iron thiocyanate FM4-64 Data Sheet complicated ion ([Fe(SCN)n]m) with Fe.
