Its matched againstdaapplying GO term search by means of transcripts with BLAST hits matched against NR NR tabase. We We detected important similarity withtotal ofof 57,106 (31 ) transcripts. The GO database. detected substantial similarity having a a total 57,106 (31 ) transcripts. The GO evaluation revealed 38,265 GO outcomes for biological processes (20.2 ), 36,046 for molecular evaluation revealed 38,265 GO outcomes for biological processes (20.two ), 36,046 for molecular functions (19.1 ) and 35,909 for cellular elements (19.0 ). A considerable proportion functions (19.1 ) and 35,909 GO GO for cellular elements (19.0 ). A considerable proportion annotated transcripts in biological process had been assigned to cellular component from the with the annotated transcripts in biological approach were assigned to cellular element MS37452 Histone Methyltransferase biogenesis (GO:0044085) and cellular localization (GO: 0051641) (Figure three). biogenesis (GO:0044085) and cellular localization (GO:0051641) (Figure three).Figure 3. GO functional classification assigned one of the most percentage with the annotated transcripts to cellular element Figure three. GO functional classification assigned the most percentage from the annotated transcripts to cellular component biogenesis term forfor biological course of action; cytosol for cellular component; and compact molecule binding term for molecular biogenesis term biological method; cytosol term term for cellular element; and small molecule binding term for function, respectively.respectively. Evaluation was with theout with system forprogram for theurchin (L. albus) Antibiotic| reference molecular function, Evaluation was carried out carried WEGO the WEGO the edible sea edible sea urchin (L. albus) transcriptome. reference transcriptome.For cellular elements and molecular functions, quite a few annotated transcripts had been For cellular components and molecular functions, a number of annotated transcripts had been allocated to cytosol (GO: 0005829) and nucleoplasm (GO:0005654); and nucleotide binding allocated to cytosol (GO:0005829) and nucleoplasm (GO:0005654); and nucleotide binding (GO:0000166), nucleoside phosphate binding (GO:1901265), and little molecule binding (GO:0000166), nucleoside phosphate binding (GO:1901265), and modest molecule binding (GO: 0036094) terms, respectively (Figure three).three). Based sequence homology, 32,231 sequences (GO:0036094) terms, respectively (Figure Based on on sequence homology, 32,231 sequences had been classified into 25 functional categories (Figure four). The represented categories had been classified into 25 functional categories (Figure 4). Essentially the most most represented categories were General Functional Predictionfollowed by Signal Signal Transduction. These had been Basic Functional Prediction only, only, followed by Transduction. These final results results indicate that we generated a reference transcriptome edible sea urchin based on indicate that we generated a reference transcriptome for the for the edible sea urchin based on 91,119,300 high-quality reads that assembled de novo into 185,239 transcripts with 91,119,300 high-quality reads that assembled de novo into 185,239 transcripts with an N50 of 1769 bp and 38.8 38.8 GC content material. an N50 of 1769 bp and GC content material.Biology 2021, ten, x Biology 2021, 10,7 of 20 7 ofFigure four. The Common Functional Prediction only was by far the most represented category for the 25 cluster of orthologous Figure 4. The General Functional Prediction only was by far the most represented category for the 25 cluster of orthologous groups (COG) functional classif.
