Ations. The samples have been collected straight from the milk cooling tank in each farm; all tanks had one or a lot more agitators which stirred the milk continuously until the cover in the tank was raised for sample collection. Milk samples were collected by Tartrazine supplier sterile plastic single-use pipettes which have been immersed into the tank to withdraw the samples. A total of 4 samples have been collected from the milk tank of every flock, and also a new pipette was utilized for each and every sample. Instantly soon after collection, samples were transferred into sterile plastic Universal-type vials. Samples had been packed at 0.0 to 4.0 C and transported for laboratory examinations [4].Biology 2021, 10,3 ofBiology 2021, 10,3 ofsamples had been transferred into sterile plastic Universal-type vials. Samples were packed at 0.0 to 4.0 and transported for laboratory examinations [4].Figure 1. Place of 325 sheep flocks about Greece, which have been visited for bulk-tank milk sampling. Figure 1. Location of 325 sheep flocks around Greece, which had been visited for bulk-tank milk sampling.two.two. Laboratory Examinations 2.2. Laboratory Examinations On the 4 milk samples collected from the milk tank of every flock, two samples have been Of your 4 milk samples collected from the milk tank of each flock, two samples were utilized for somatic cell Bryostatin 1 Autophagy counts (SCC) and milk composition measurement; the otherotherwere utilized for somatic cell counts (SCC) and milk composition measurement; the two two were used for the bacteriological examinations. subsamples were had been createdprocessed used for the bacteriological examinations. Two Two subsamples created and and processed from every from the four samples, such that eachtest was performed performed(each from each and every of the 4 samples, such that each and every separate separate test was four times 4 occasions (each 1 in diverse subsamples). 1 in various subsamples). SCC and milk composition measurement were performed within four h of collection, while SCC and milk composition measurement have been performed inside four h of collection, while bacteriological examinations began within 24 h of sample collection [4]. Bacteriobacteriological examinations began within 24 h of sample collection [4]. Bacteriological logical examinations from every ofrelevant subsamplessubsamples included total bacterial examinations from each and every in the 4 the 4 relevant incorporated total bacterial counts (TBC), counts (TBC), performed by employing theprocedures described by Laird et al. [12], and performed by employing the standardized standardized procedures described by Laird et al. [12],on Staphylococcus-selective medium (Mannitol salt agar; BioPrepare Microbiology, culturing and culturing on Staphylococcus-selective medium (Mannitol salt agar; BioPrepare Microbiology, Athens, Greece) for aerobic incubation at 37 forno development, media Athens, Greece) for aerobic incubation at 37 C for 48 h; if there was 48 h; if there was no development, media were re-incubated to get a further 24 h. Immediately after completion ofwithdrawal for have been re-incubated for a further 24 h. After completion of sample aliquot sample aliquot withdrawal for examination, the examination, with the respective samplesrespective samples microbiological microbiological temperature the temperature of your was measured and was measured and was identified to never ever exceed 3.8 . found to never ever exceed three.eight C. Bacterial isolation and initial identification by implies Gram staining and evaluation Bacterial isolation and initial identification by signifies ofof Gram staining and ev.
