Treated photoaging HDFOn the basis of the document and previous experiment
Treated photoaging HDFOn the basis of the document and previous experiment, injected 50 ng/ml,100 ng/ml,150 ng/ml final concentraion of the SFRP2 into ADSC-CM co-cultured photoaging HDF, 37 5 CO2 constant temperature cultured,after 48 h, measureed wnt3a and -catenin expression in mRNA level.Statistical analysisData were collected from at least three independent experiments. One-way ANOVA test, followed by paired t-test, was used for statistical analysis among different groups. P < 0.05 was considered significant, p < 0.01 was considered significant obviously.Competing interests The authors declare that they have no competing interest. Authors' contributions XX carried out most of the experiments and drafted the manuscript; WHY carried out the in vivo studies; ZY and LYQ assisted in the in vitro and vivo studies; LY participated in the design of the studies; JJD and TK participated in the design of the studies; WCT participated in the design of the studies; LXY conceived the studies, coordinated the experiments and was involved in the drafting the manuscript and revising it. All authors read and approved the final manuscript. PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28549975 Acknowledgements We would like to express our gratitude to Yao Yao, who is from Da Lian Medical University, for his contribution to animal work, and we would also like to thank Ruiting Sun, who is from An Hui Medical University, for her contribution to analysis and interpretation of ADSCs with flow cytometry.Xu et al. Cell Bioscience 2014, 4:24 http://www.cellandbioscience.com/content/4/1/Page 11 of22. Alexiades-Armenakas MR, Dover JS, Arndt KA: Fractional laser skin resurfacing. J Drugs Dermatol 2012, 11(11):1274?287. 23. Tierney EP, Hanke CW: Fractionated carbon dioxide laser treatment of photoaging: prospective study in 45 patients and review of the literature. Dermatol Surg 2011, 37(9):1279?290. 24. Carniol PJ, Harirchian S, Kelly E: Fractional CO2 laser resurfacing. Facial Plast Surg Clin North Am 2011, 19(2):247?51. 25. Tenna S, Cogliandro A, Piombino L, Filoni A, Persichetti P: Combined use of fractional CO2 laser and radiofrequency waves to treat acne scars: a pilot study on 15 patients. J Cosmet Laser Ther 2012, 14(4):166?71. 26. Zhou BR, Xu Y, Guo SL, Wang Y, Zhu F, Permatasari F, Wu D, Yin ZQ, Luo D: The effect of conditioned media of adipose-derived stem cells on wound healing after ablative fractional carbon dioxide laser resurfacing. RG7800 web Biomed Res Int 2013, 2013:519126. 27. Berneburg M, Plettenberg H, Krutmann J: Photoaging of human skin. Photodermatol Photoimmunol Photo Med 2000, 16(6):239?44. 28. Fisher GJ, Kang S, Varani J, Bata-Csorgo Z, Wan Y, Datta S, Voorhees JJ: Mechanisms of photoaging and chronological skin aging. Arch Dermaml 2002, 138(11):1462. 29. Berrnstein EF, Andersen D, Zelickson BD: Laser resurfacing for dermal photoaging. Clin Plast Surg 2000, 27(2):221. 30. Rehman J, Traktuev D, Li J, Merfeld-Clauss S, Temm-Grove CJ, Bovenkerk JE, Pell CL, Johnstone BH, Considine RV, March KL: Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation 2004, 109:1292?298. 31. Watson RE, Griffiths CE: Pathogenic aspects of cutaneous photoaging. J Cosmet Dermatol 2005, 4:230?36. 32. Le Pillouer-Prost A: Fibroblasts: what’s new in cellular biology? J Cosmet Laser Ther 2003, 5(3?):232?38. 33. Park BS, Kim WS, Choi JS, Kim HK, Won JH, Ohkubo F, Fukuoka H: Hair growth stimulated by conditioned medium of adipose-derived stem cells is enhanced by hypoxia: evidence of incre.
