FKBP motif or a domain of the PI3K homolog FRAP. Rev protein Functional Analysis of HIV-1 Rev Oligomerization 8 Functional Analysis of HIV-1 Rev Oligomerization RevSLT40, the data obtained in the present study provide to our knowledge the so far most definitive experimental evidence that multimer-formation of Rev is essential for nuclear export of Rev-containing RNP complexes. Clearly, dimer induction by fusion of heterologous dimerization motifs to the amino terminus of the otherwise multimerizationdeficient mutant RevSLT40 could not have been expected to restore Rev activity to its full extent. Nevertheless, trans-activation activity was routinely restored to levels ranging from 40% to 68% of Rev wildtype activity, depending on the assay used. This is in good agreement with our quantitative FRET measurements, in which co-expression of CFP and YFP labeled ZipRevSLT40 resulted in a dimerization capacity of about 52% when compared to the wildtype protein. Although in our experiments the formation of a Rev dimer was sufficient to drive to a detectable extent virus replication, it is still unknown how many Rev molecules have to be assembled on the RRE for maximal activity. A large body of previous biochemical and cell-based studies resulted in a model of Rev:RRE interaction, in which the initial binding of a Rev monomer to the SLIIB highaffinity binding site triggers the recruitment of additional Rev monomers by cooperative Rev:Rev interaction, resulting in the occupation of secondary low-affinity sites in the RRE. Careful protein titration studies in combination with functional assays indicated already previously that two monomeric Rev proteins bound to the RRE results in measurable biological activity. Similar data were also reported in an independent functional study using a heterologous Rev-responsive reporter construct. Considerable Rev activity was observed when the RRE was replaced by phage MS2 operator RNA and Rev was expressed as fusion to MS2 coat protein. Apparently, upon RNA binding the Rev/MS2 protein dimerized via its MS2 moiety, resulting in Rev trans-activation. Rev activity then further increases with the number of Rev molecules that are recruited onto the RRE. Importantly, this sequential model of Rev:RRE interaction has been recently confirmed and refined by employing single-molecule fluorescence spectroscopy, directly demonstrating that Rev indeed assembles on RRE RNA one molecule after another. HIV-1 Rev trans-activation induces the Foretinib transit from the early phase to the late phase of viral mRNA expression, a process that depends on Rev’s nuclear mRNA export activity. The transport of Rev-containing RNP complexes across the nuclear envelope is mediated by the cellular export receptor CRM1 and associated factors. CRM1 generally mediates the nuclear export PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/22189542 of non-coding RNA, ribosomal subunits and numerous proteins and therefore specifies a rather uncommon route for the nuclear export of mRNA. By using the highly specific CRM1 inhibitor leptomycin B, the analysis 
of Rev shuttling clearly demonstrated nuclear export of the oligomerization-deficient RevSLT40 protein via the CRM1 pathway. In contrast, however, RevSLT40 was unable to mediate the export of RRE-containing transcripts, an activity that was restored by dimer formation. This finding may suggest that a single CRM1 molecule is sufficient to mediate the nuclear export of a monomeric Rev protein in absence of RRE RNA-binding. However, multiple Rev proteins and, due to R
