lobal statistical analysis suggests that the experimentally confirmed imprinted genes affected by SYT-SSX1 in all MSCs include H19, CDKN1C, DLK1, DIRAS3 and IGF2 but that, remarkably, most of them show different expression profiles in the four cell batches, namely, strong induction in batch 4, more moderate induction in batches 1 and 2 and no induction in batch 3. These RRx-001 observations were validated by real time PCR for H19 and IGF2. Single batch gene expression analysis according to Gene Ontology BML-210 annotation, revealed remarkable variation among the batches. The GO term nervous system development was also over-represented in batch 2 but not in batch 1 or 3, consistent with SYTSSX1- mediated pressure toward neuronal differentiation in some MSC populations only. Single population analysis also suggested a variable effect of SYT-SSX on the expression of genes encoding proteins that constitute components of the extracellular matrix, mediate cellextracellular matrix interactions, and participate in vascular development, angiogenesis, tissue remodeling and cell motion. In batch 3, GO term analysis suggests inhibition of expression of these genes. Similarly, in batch 1, the list of repressed genes included over-representation of the GO terms extracellular matrix, extracellular region, proteinaceous extracellular matrix, cell adhesion, integrin complex, integrin-mediated signaling pathway. By contrast, in batches 2 and 4, the GO terms cell adhesionand extracellular matrix were over-represented in the list of induced genes. Expression of these categories of proteins is important for the biological function of both normal and cancer stem cells, the former requiring release from their niche in the bone marrow in order to be recruited to target tissues where they undergo in situ differentiation and contribute to tissue regeneration, the latter using similar mechanisms to disseminate and form metastases. CXCR4 and integrins are among the main effectors of these functions, and induction of CXCR4 was observed in populations 2 and 4. Because the level of SYT-SSX expression was comparable in all four MSC populations, and because each MSC population was subjected to identical cultur
