Quantum mechanical calculation is also conducted for analysis of electrostatic characteristics of compounds. Inspection of the molecular electrostatic potential surfaces and frontier molecular orbitals successfully explained their significant role in driving the inhibitor to adopt a suitable bioactive conformation oriented in the active site of enzyme. In general, this study is used as example to illustrate how multiple pharmacophore approach can be useful in identifying structurally diverse hits which may bind to all possible bioactive conformations available in the active site of enzyme. The present study may lead to the knowledge of chemical properties which are likely to improve activity of already known chymase inhibitors and may also allow the modification of the structure of new chemical entities for the improved bioavailability. The application of multiple pharmacophore-based VS can also be extended to the development of fast-follower drugs, where more than one high-quality crystal structures of the target in complex with potent ligands are already available. Therefore, the multiple pharmacophore modeling approach can be very useful in virtual screening of any chemical database for the development of new potent inhibitors for the enzyme. Recent studies suggest that sphingolipids can induce phenotypes such as proliferation, adhesion and angiogenesis-the hallmarks in tumor growth and metastasis. Application of drugs which inhibit glycosphingolipid synthesis provide an opportunity to examine the role of these compounds in animal models of human disease. Here we demonstrate that by linking glycosphingolipid synthesis and its inhibition in a mouse model of renal cancer, it is possible to observe the footprint of interactions between drug and glycosphingolipid metabolizing enzymes and to predict the onset of disease/tumor progression and tumor regression. Blocking the ONO-4059 (hydrochloride) glycosylation of ceramide to treat cancer has been documented in cell and in animal models. Tumors (+)-JQ-1 require new blood vessel formation from pre-existing ones and vascular endothelial growth factor plays a critical role in inducing angiogenesis in a variety of tumors. Therefore, we rationalized that targeting endothelial cells that line the tumor blood vessels, which
