However, the 72h-recovery in a drug-free medium resulted in almost complete restoration of normal values, arguing in favor of reversible covalent association between the DNMTs and the zebularine-containing DNA. Very importantly, there seemed to be a negative interplay between HATs and Hdac1/HDACs the newly synthesized HAT, such as Cdyl, might be one direct regulator of HDAC degradation, so that the latter would provide a necessary HDACfree environment for histone hyperacetylation. Anyhow, the HAT in charge of histone hyperacetylation still requires inspection. Taken together, we put forward a working hypothesis of reprogramming in spermiogenesis as follows After meiosis, transcription restarted in round spermatids, during this period, some essential and special HATs were generated. Next, HDACs catalyzed the deacetylation of histones, which in turn gave rise to the extensive dissociation of CAFs. Then the transcription finally stopped. The mechanism involved in this erasure process might be universal in gametogenesis. In the meanwhile, the MCE Company 1000669-72-6 gradually accumulated HATs guided the degradation of HDACs, in that case they could induce the histone hyperacetylation in elongating spermatids. Soon the AcH signal recruited remodeling factors as BRDT to execute the histone substitution and nuclear condensation. This elongating program was spermatogenic-specific. During the reprogramming in spermiogenesis, the regulation of histone acetylation might be central of the controlling network, the balance between HATs and HDACs played an important role. These hypotheses need further investigations. Curcumin is extracted from the plant Curcuma longa, has been used as food addictive for thousands of years in Asia. Most recently, it is proved to have a wide range of pharmacological activities, including antioxidant, anti-inflammatory, antiviral, antibacterial, 1092351-67-1 antifungal, and anticancer, as well as a potential that against diverse malignant diseases, diabetes, allergies, arthritis, Alzheimers disease, and other chronic illnesses. Curcumin has been listed as third generation cancer chemopreventive agent by the Institution of Cancer Chemoprevention, NCI, NIH of United States. The effects of Curcumin are mediated through a very complex network, the regulation of various transcription factors, growth factors, inflammatory cytokines, protein kinases, and other enzymes, whereas we especially concern about its property of HAT inhibitor. Our current knowledge about Curcumin is mainly from researches based on disease models. For example, Curcumin displayed a protective function on testicular tissues under various pathological conditions.
